受体相互作用蛋白1(RIP1)对BCG诱导小鼠巨噬细胞凋亡的调控作用

doi:10.11843/j.issn.0366-6964.2019.03.020

Abstract

Abstract:

The purpose of this study was to explore the effect of RIP1 on apoptosis of RAW264.7 cell by assessment the apoptosis related indicators of RAW264.7 cell treated with RIP1 RNAi vector or/and BCG infection. The RIP1 adenovirus RNAi vector was constructed and transfected into BCG-infected RAW264.7 cell line. Flow cytometry was used to detect the apoptotic rate, mitochondrial membrane potential, cell reactive oxygen species and cell cycle of RAW264.7 cell. The expression of RIP1 and apoptosis associated proteins were examined by Western blot. The results showed that BCG-infection can significantly induce RAW264.7 cells apoptosis, which was accompanied with up-regulation of PIP1 protein. In addition, a RIP1 adenovirus RNAi vector showed an ability to reduce the BCG-induced macrophage apoptosis along with an increased mitochondrial membrane potential and reduced reactive oxygen species (ROS). It also down-regulated Bax expression and up-regulated Bcl-2 expression of RAW264.7 cell infected with BCG. Intriguingly, cell cycle of RAW264.7 cell was arrested in G₁ phase. In conclusion, RIP1 involves in BCG-induced apoptosis by down-regulating mitochondrial membrane potential, increasing the production of ROS and the ratio of apoptosis-related proteins (Bax/Bcl-2) and arresting the cell cycle in G₁ phase in BCG-infectied RAW264.7.

CLC Number:

R52
S855

FANG Shu, ZHANG Jiamei, YANG Yi, HAN Lu, MA Chenjie, WU Xiaoling, DENG Guangcun. Regulation of Receptor-interacting Protein 1 on Apoptosis of Macrophage Induced by Bacillus Calmette-Guerin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 645-653.

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Regulation of Receptor-interacting Protein 1 on Apoptosis of Macrophage Induced by Bacillus Calmette-Guerin

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